ABSTRACT
Objective: To establish the HPLC characteristic spectrum of Lygodii Herba from different habitats in Guangxi Province, and to evaluate the difference of components in different parts of medicinal materials. Methods: HPLC was performed on Waters symmetry C18 (250 mm × 4.6 mm, 5 μm) column, with the mobile phase of acetonitrile-0.2% phosphoric acid at flow rate of 1.0 mL/min; Detection wavelength was 354 nm; The column temperature was 30 ℃ and the sample size was 20 μL. Twelve batches of Lygodii Herba samples were determined and the characteristic spectrum of those were established, and the content of rutin, isoquercetin, and astragalin were determined to evaluate the difference of chemical components in different parts of medicinal materials. Results: There were seven characteristic peaks identified in the characteristic spectra of Lygodii Herba samples. Peak 1 was caffeic acid, peak 2 was Rutin, peak 3 was Isoquercitrin and peak 5 was astragalin. The similarities of 11 batches of samples were proved to be higher than 0.900 and one batch of them was proved to be less than 0.900. The chemical component of stem was richer than that of leaves of Lygodii Herba, and the content of the component was higher in the stem than that of the leaves. Conclusion: The method is simple, accurate, and reproducible, which can provide the scientific evidence for controlling the internal quality standards effectively. The preferred harvest season of Lygodii Herba is autumn and winter.
ABSTRACT
Objective: To establish a method for the determination of chlorogenic acid, caffeic acid, and 6-O-p-coumaroyl-D-glucopyranose in Lygodii Herba. Methods: The determination was developed on Phenomenex C18 column (250 mm × 4.6 mm, 5 μm), methanol-acetonitrile-1% glacial acetic acid (5:5:90) was used as mobile phase, the detective wavelength was 325 nm, and the column temperature was 35 ℃. Results: The linear ranges of chlorogenic acid, caffeic acid, and 6-O-p-coumaroyl-D-glucopyranose were 0.368-2.760, 0.064-0.480, and 0.102-0.765 μg, respectively. The average recoveries were 101.48% (RSD = 1.44%), 99.56% (RSD = 0.92%), and 101.73% (RSD = 1.62%), respectively. Conclusion: The method is simple, accurate, and highly reproducible, which could be used for the quality control of Lygodii Herba.
ABSTRACT
Objective: To expound the origin of Lygodii Herba widely used in the folk medicine. Methods: The records and drawings of Lygodii Herba were studied, the origin of Lygodii Herba in the field was investigated, the specimens of Lygodii Herba in the producing area were collected and checked, and the specialists of taxology were consulted. Results: Now in the market the origins of Lygodii Herba were the aerial parts of Lygodium japonicum, L. microphyllum, and L. fiexuosum. The aerial parts of L. yunnanense, L. polystachyum, and L. salicilofolium in the same family were also used in some places. TLC analysis showed there existed main spots in different origins of Rosae Laevigatae Radix and the commercial herb samples. Conclusion: The investigation could provide the reference of medicinal material supply for the pharmaceutical factory, and insure the quality of Lygodii Herba.